C7947-97
Clone Type
PolyclonalHost
GoatSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
ECrossreactivity
Ca HuAccession #
NP_001008393.1, NP_005799.2Gene ID
10217Shipping Temp
Blue IceStorage Temp
-20°CGoat Anti-CTDSPL (CTD Small Phosphatase-like Protein, CTDSP-like, Carboxy-terminal Domain RNA Polymerase II Polypeptide A Small Phosphatase 3, NIF-like Protein, Nuclear LIM Interactor-interacting Factor 1, NLI-interacting Factor 1, Protein YA22, hYA22, RBSP3, Small C-terminal Domain Phosphatase 3, SCP3, Small CTD Phosphatase 3, C3orf8, NIF1, NIFL, SCP3, YA22)
Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:32,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to QCNVSLKKQRSRS, from CTDSPL, at the internal region of the protein (NP_001008393.1, NP_005799.2).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes CTDSPL. Species sequence homology: Canine and human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Kashuba VI, Li J, Wang F, Senchenko VN, Protopopov A, Malyukova A, Kutsenko AS, Kadyrova E, Zabarovska VI, Muravenko OV, Zelenin AV, Kisselev LL, Kuzmin I, Minna JD, Winberg G, Ernberg I, Braga E, Lerman MI, Klein G, Zabarovsky ER. RBSP3 (HYA22) is a tumor suppressor gene implicated in major epithelial malignancies. Proc Natl Acad Sci USA. 2004 Apr 6;101(14):4906-11. Epub 2004 Mar 29.USBio References
No references available