Technical Data

C7948-25
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Accession #
AAQ88749
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-CTRP5 (C1q Tumor Necrosis Factor alpha Related Protein 5)

Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines (reviewed in 1). One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-α- related proteins (CTRPs) 1-7 (2). Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues (3). These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene (4). CTRP5 has been suggested to be involved in age-related macular degeneration (5).

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Caco-2 cell lysate
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide CTRP5, 15aa near the center of human CTRP5 (Genbank accession No. AAQ88749).
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.|
Purity
Purified by immunoaffinity chromatography.|
Specificity
Recognizes human CTRP5.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Fantuzzi G. J. Allergy Clin. Immunol. 2005; 115: 911-9. 2. Tsao T-S, Lodish HF, and Fruebis J. Euro. J. Pharmacol. 2002; 440: 213-21. 3. Wong GW, Wang J, Hug C, et al. Proc. Natl. Acad. Sci. USA 2004; 101: 10302-7. 4. Shapiro L and Scherer PE. Curr. Biol. 1998; 8: 335-8.
USBio References
No references available
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