C7949-13D
Clone Type
PolyclonalHost
GoatSource
MouseIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
Bo Ca Hu Mo RtAccession #
NP_003582.2Gene ID
8453, 71745, 361258Shipping Temp
Blue IceStorage Temp
-20°CGoat Anti-Cullin-2 (CUL-2, CUL2, Cullin 2)
CUL-2 is a member of the family of human cullin genes (CUL-1, -2, -3, -4a, -4b, and -5), homologous to the S. cerevisiae cdc53 gene. CUL-1 forms a complex with human p19skp1 and F box protein p45skp2. Together with cdc53 (known as CulA), the three subunits, form a ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. In contrast to CUL-1, CUL-2 does not associate with p19skp1 and p45skp2. Instead, CUL-2 forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongain C, Elongin B and VHL. The function of this association remains unknown.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:8000 Western Blot: 1-3ug/ml, lysates of cell line NIH3T3 observed on ~100kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-QYIERSQASADE, from mouse CUL2, at C-terminal (NP_003582.2).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse CUL2. Species sequence homology: Bovine, canine, human and rat.
