Rabbit Anti-Cullin 7 (CUL-7, p185, KIAA0076, Cullin homolog 7, p180)
Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 7 (CUL 7) is a component of SCF-like E3 ubiquitin ligase complex and may play a role in the degradation of proteins involved in endothelial proliferation and/or differentiation. Cullin 7 is a new oncogene that cooperates with Myc in transformation by blocking Myc-induced apoptosis in a p53-dependent manner.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:100 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide (RGVPYASCTATQSFSTF) corresponding to aa1681-1697 of human Cullin 7
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human CUL 7
