Rabbit Anti-CXCL1 (Gro, KC)
CXCL1, also known as Gro and KC., is an 8kD cytokine which is expressed predominantly by monocytes after activation, but it also expressed by fibroblasts, endothelial cells, synovia cell and several tumor cell lines. CXCL1 promotes neutrophil chemotaxis and degranulation.
Applications
Suitable for use in ELISA, Western Blot and Functional Assays. Other applications not tested.
Recommended Dilutions
ELISA: 0.5-2ug/ml Western Blot: 0.1-0.2ug/ml Functional Assays: 5-10ug/ml Optimal working dilutions to be determined by the researcher.
Recommended Secondary Reagents
I1904-40J: IgG, Fc (HRP) Pab Gt xRb Sheep anti Rabbit IgG (HRP)
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O . Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant rat CXCL1.
Form
Supplied as a lyophilized powder from PBS, pH 7.4. No preservatives added. Reconstitute with 100ul sterile ddH2O.
Purity
Purified by affinity chromatography.
Specificity
Recognizes rat CXCL1.
