Rabbit Anti-Cyclin E (Not for Export EU)
Cyclin E is a member of the cyclin family. Cyclin E binds to G1 phase Cdk2, which is required for the transition from G1 to S phase. The Cyclin E/CDK2 complex phosphorylates p27Kip1 (an inhibitor of Cyclin D), tagging it for degradation, thus promoting expression of Cyclin A, allowing progression to S phase.
Applications
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
ELISA: 1:100,000 Immunoprecipitation: 1:100 Western Blot: 1:2000-1:10,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide corresponding to the C-terminal of human Cyclin E.
Form
Supplied as a liquid, 0.01% sodium azide.
Specificity
Recognizes human Cyclin E at 45kD. IEP Assay shows a single precipitin arc against source serum protein and IgG. No reaction was observed against other related cyclins.
