Goat Anti-Cyclophilin A (Peptidyl-prolyl Cis-trans Isomerase A, PPIase A, Cyclosporin A-binding Protein, Rotamase A, PPIA, CYPA)
Cyclophilin A, also called Peptidylprolyl Isomerase A, PPIA, CYPA, and CYPH, was originally characterized for its ability to catalyze the transition between cis-and trans-proline residues critical for proper folding of proteins. Cyclophilin is also incorporated into many viruses, including HIV1, where it has been speculated to be involved in functions such as viral assembly and infectivity. The immunosuppressive activity of cyclosporins has been correlated with their ability to form complexes with cyclophilins that inhibit calcineurin phosphatase activity and prevent incorporation of cyclophilin into viral particles. The cyclosporin/cyclophilin complex selectively binds and inactivates calcineurin, making it a useful inhibitor for studying calcineurin activity.
Applications
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.1ug/ml (HeLa cervical epithelial carcinoma, C2C12 mouse myoblast, and Nb2-11 rat lymphoma cell lines) Immunocytochemistry: 5-15ug/ml (Immersion fixed PANC-1 human pancreatic carcinoma cell line) Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Met1-Glu165 from human Cyclophilin A expressed in E.coli.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Cyclophilin A. Species Crossreactivity: Mouse and rat.
