C8950-11
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Rabbit Anti-Cystatin C (AD8, Amyloid Angiopathy and Cerebral Hemorrhage, Cst3, Cystatin 3, Gamma Trace, HCCAA, Neuroendocrine Basic Polypeptide, Post gamma Globulin, ARMD11, MGC117328, OTTHUMP00000030440, OTTHUMP00000164181, bA218C14.4)
Cystatin C is a non-glycosilated basic single-chain protein consisting of 120 amino acids with a molecular weight of 13.36kD and is characterized by two disulfide bonds in the carboxy-terminal region. It belongs to the cystatins superfamilly which inactivates lysosomal cysteine proteinases, e.g. cathepsin B, H.K, L and S. Imbalance between Cystatin C and cysteine proteinases is associated with inflammation, renal failure, cancer, Alzheimer's di-sease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy. Its increased level has been found in patients with autoimune diseases, with colorectal tumors and in patients on dyalisis. Serum Cystatin C seems to be better marker of glomerular filtration rate than creatinine. On the other hand, low concentration of Cystatin C presents a risk factor for secondary cardiovascular events.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Cystatin C from human urine
Form
Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile dH2O.
Purity
Purified by Immunoaffinity chromatography
Specificity
Recognizes human Cystatin C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1.Danjo A, Yamaza T, Kido MA, Shimohira D, Tsukuba T, Kagiya T, Yamashita Y, Nishijima K, Masuko S, Goto M, Tanaka T . Cystatin C stimulates the differentiation of mouse osteoblastic cells and bone formation. Biochem Biophys Res Commun. Aug 17;360(1):199-204 (2007). 2. Nedelkov D, Nelson RW . Delineating protein-protein interactions via biomolecular interaction analysis-mass spectrometry. J Mol Recognit . Jan-Feb;16(1):9-14 (2003). 3. Nedelkov D, Nelson RW. Design and use of multi-affinity surfaces in biomolecular interaction analysis-mass spectrometry (BIA/MS): a step toward the design of SPR/MS arrays. Mol Recognit. Jan-Feb;16(1):15-9 (2003). 4. Konduri SD, Yanamandra N, Siddique K, Joseph A, Dinh DH, Olivero WC, Gujrati M, Kouraklis G, Swaroop A, Kyritsis AP, Rao JS. Modulation of cystatin C expression impairs the invasive and tumorigenic potential of human glioblastoma cells. Oncogene. Dec 12;21(57):8705-12 (2002)USBio References
No references available