C8950-11A
Clone Type
PolyclonalHost
SheepSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Sheep Anti-Cystatin C (AD8, Amyloid Angiopathy and Cerebral Hemorrhage, Cst3, Cystatin 3, Gamma Trace, HCCAA, Neuroendocrine Basic Polypeptide, Post gamma Globulin, ARMD11, MGC117328, OTTHUMP00000030440, OTTHUMP00000164181, bA218C14.4)
Cystatin C is a non-glycosilated basic single-chain protein consisting of 120 amino acids with a molecular weight of 13.36kD and is characterized by two disulfide bonds in the carboxy-terminal region. It belongs to the cystatins superfamilly which inactivates lysosomal cysteine proteinases, e.g. cathepsin B, H.K, L and S. Imbalance between Cystatin C and cysteine proteinases is associated with inflammation, renal failure, cancer, Alzheimer's di-sease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy. Its increased level has been found in patients with autoimune diseases, with colorectal tumors and in patients on dyalisis. Serum Cystatin C seems to be better marker of glomerular filtration rate than creatinine. On the other hand, low concentration of Cystatin C presents a risk factor for secondary cardiovascular events.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stabillty
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Cystatin C from human urine
Form
Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile dH2O. Let the lyophilized pellet dissolve completely.
Purity
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human Cystatin C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Page M.K. et al.: . Clinical value of cystatin C determination. Clinica Chim. Acta 297, 67-72 |(2000) . |2. Pirttila TJ, Pitkanen A. Cystatin C expression is increased in the hippocampus following |photothrombotic stroke in rat. Neurosci Lett. 2006 Mar 6;395(2):108-13. Epub 2005 Nov 23. |3. Bokenkamp A, Ciarimboli G, Dieterich C. Cystatin C in a rat model of end-stage renal failure. |Ren Fail. 2001 May-Jul;23(3-4):431-8. |4. Nagai A, Ryu JK, Kobayash S, Kim SU. Cystatin C induces neuronal cell death in vivo. Ann N Y |Acad Sci. 2002 Nov;977:315-21. |5. Xu L, Sheng J, Tang Z, Wu X, Yu Y, Guo H, Shen Y, Zhou C, Paraoan L, Zhou J. Cystatin C |prevents degeneration of rat nigral dopaminergic neurons: in vitro and in vivo studies. Neurobiol |Dis. 2005 Feb;18(1):152-65. |6. Price Ch.P., Finney H. Developments in the assessment of glomerular filtration rate. Clinica |Chimica Acta 297, 55-66 (2000) . |7. Ono S. et al. Increased cystatin C immunoreactivity in the skin in amyotrophic lateral sclerosis. |8. Nicklin M.J.H. and Barrett A.J. Inhibition of cysteine proteinases and dipeptidyl peptidase 1 by |eggwhite cystatin. Biochem J. 223, 245-253 (1984) . |9. Lindahl P., Abrahamson M., Bjork L.: . Interaction of recombinant human cystatin C with the |cysteine proteinase papain and actinidin. Biochem. J. 281, 49-55 (1996) . |10. Roald AB, Aukland K, Tenstad O. Tubular absorption of filtered cystatin-C in the rat kidney. Exp |Physiol. 2004 Nov;89(6):701-7. Epub 2004 Aug 24.USBio References
No references available