Technical Data

C9020-01
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Isotype
IgG
Grade
Affinity Purified
Applications
IHC IP WB
Crossreactivity
Bo Mo Rt Xe
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Cysteine String Protein (CSP, DnaJC5)

Cysteine string protein (CSP) is a 34kD rat brain protein localized to the cytoplasmic surface of the synaptic vesicle, possibly via palmitoylation (1,2). CSP homologs are localized to the nervous system of Drosophila and to cholinergic vesicles of Torpedo (3,4). The amino terminus of CSP contains the J domain, a consensus sequence found on a DnaJ like protein which is a member of the heat-shock protein family (5). Since heat shock proteins serve as chaperones that participate in protein folding and protein complex formation, CSP is believed to perform similar functions in the assembly of synaptic vesicle proteins which are essential to membrane trafficking. Evidence for a role of CSP in presynaptic neurotransmitter release has come from genetic and physiological study in which the Drosophila CSP null mutant fails to elicit synaptic potential upon high frequency stimulation (6). CSP null mutants have a lethal phenotype and for the small percentage of mutant flies that survive, they become paralyzed and die at elevated temperature (7).

Applications
Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Western Blot (Colorimetric): 1:2000 Immunoprecipitation: 0.5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide mapping to a fragment of residues in the C-terminal region of rat CSP.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat CSP. Species Crossreactivity: mouse, bovine and xenopus

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Baun, J.E.A. and Scheller, R. (1995) Neuropharmacology 34: 1361-1369. Gundersen, C.B., Mastrogiacomo, A., Faull, K., & Umbach, J.A. (1994) J. Biol. Chem. 269: 19197-19199. Mastrogiacomo, A., Parsons, S.M., Zampighi, G.A., Jenden, D.J., Umbach, J.A. & Gundersen, C.B. (1994) Science 263: 981-982. Zinsmaier, K.E., Hofbauer, A., Heimbeck, G., Pflugfelder, G.O., Buchner, S., & Buchner, E. (1990) J. Neurogenet. 7: 15-29. Georgopoulos, C. & Welch, W.J. (1993) Ann. Rev. Cell Biol. 9: 601-634. Umbach, J.A., Zinsmaier, K.E., Eberle, K.K., Buchner, E., Benzer, S., & Gundersen, C.B. (1994) Neuron 13: 899-907. Zinsmaier, K.E., Eberle, K.K., Buchner, E., Walter, N., & Benzer, S. (1994) Science 263: 977-980. Thomas, D.D.H., Taft, W.B., Kaspar, K.M., Groblewski, G.E. (2001) J Biol Chem 276(31): 28866-28872. Sakisaka, T., Meerol, T., Matteson, J., Plutner, H., Balch, W.E. (2002) EMBO J 21(22): 6125-6135.
USBio References
No references available
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