Rabbit Anti-Cytochrome P450 Aromatase (P450 Arom)
ARO1, CYAR, CYP19, CYP19A1, CYPXIX, Cytochrome P450 19A1, Estrogen synthetase, P-450AROM|
Aromatase is a key enzyme in steroidogenesis and plays an important role in sexual differentiation, fertility, and carcinogenesis. Many environmental chemicals may influence aromatase activity and thereby disrupt endocrine function.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry, and . Other applications not tested.
Recommended Dilutions
Western Blot: 1ug/ml (ECL). A band is seen at ~55kD human brain. Immunocytochemistry/Immunofluorescence: 1:100 Observed in SH-SY-5Y cells. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa400-502 from the C-terminal domain of human aromatase protein sequence. Species Sequence Homology: 91% equine, 90% bovine, rabbit, 83% porcine, 81% sheep, goat, canine. UniProt Accession #: P11511
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Cytochrome P450 Aromatase. Species Crossreactivity: primate and rabbit
