Mouse Anti-Cytokeratin 14
Cytokeratin 14 is one of two cytokeratins that can distinguish stratifying epithelial cell types from simple epithelial cell types, being expressed by stratifying/keratinocytes cell types but not by simple epithelial cells.
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry, Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:100. Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required. Immunohistochemistry: frozen sections Immunohistochemistry (paraffin): 1:200. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer is recommended for this purpose. Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo
Recommended Positive Control Tissue
Skin
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Last 15 C-terminal residues of human cytokeratin 14 conjugated to thyroglobulin.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Ion Exchange chromatography from tissue culture supernatant.
Specificity
Recognizes human cytokeratin 14, a type I intermediate filament. Species Crossreactivity: elephant, canine, lion and porcine
