Mouse Anti-Cytokeratin 7,17
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Applications
Suitable for use in Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Immunohistochemistry (paraffin): 10ug/ml with pretreatment: 0.1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB Western Blot: 1-2ug/ml using HeLa cells as a positive control Optimal dilutions to be determined by the researcher.
Immunohistochemistry Positive Control
Human pancreas.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human cytokeratin peptides 7 and 17 (54 and 46 kD).. Species Crossreactivity: bovine and porcine. Does not react with mouse, rabbit or rat.
