Mouse Anti-Cytomegalovirus Glycoprotein B, Late Cytoplasmic Antigen (CMV gB, CMV Glycoprotein GP55, HCMV, Human Herpes virus 5, HHV-5, HHV5, UL55) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Cytomegalovirus is an enveloped double stranded DNA virus which in humans (HCMV) is commonly known as human Herpes virus 5 (HHV-5). Glycoprotein B (gB) is the main antigenic protein within the envelope of HCMV. It is required for HCMV mediated membrane fusion along with the gH / gL complex. The unprocessed precursor of HCMV gB is 906 amino acids in length, with a MW of ~100kD.
Applications
Suitable for use in FLISA, Immunofluorescence and Immunohistochemistry (frozen sections). Other applications not tested.
Recommended Dilution
Immunofluorescence: 0.02-1ug/slide; Reactive only in acetone fixed CMV infected cells or frozen sections. Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-14C: IgG, Fc (HRP) Pab Gt x Mo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt x Mo
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes cytomegalovirus glycoprotein B, a viral ligand for CD209 which allows capture of viral particles by dendritic cells. Does not react with HSV, VSV or EBV.
