Mouse Anti-D4-GDI (GDP Dissociation Inhibitor, Rho GDP-dissociation Inhibitor 2, Rho GDI 2, Ly-GDI, Rho-GDI beta, ARHGDIB, GDIA2, GDID4, RAP1GN1) (Biotin)
D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28kD mature form of D4-GDI to give a 23kD and 5kD size fragment. The 23kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Whole cell lysate from Daudi or Jurkat cells
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human D4-GDI.
Form
Supplied as a liquid in PBS, 0.02% sodium azide. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human D4-GDI at ~28kD.
