Rabbit Anti-DARPP-32, phosphorylated (Thr34) (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)
DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr-34. In contrast, when DARPP-32 is phosphorylated on Thr-75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000. Detects a band of ~32kD in rat caudate lysates. Optimal dilution determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr34 of rat DARPP-32 (KLH). Species Sequence Homology: canine, human, bovine, chicken and monkey.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 0.1% BSA, 150mM sodium chloride, 50% glycerol.
Purity
Purified by affinity chromatography.
Specificity
Recognizes endogenous levels of rat DARPP-32 when phosphorylated at threonine 34. Species Crossreactivity: mouse