Technical Data

D1499-01
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Highly Purified
Applications
E WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
4°C (-20°C Glycerol)
Rabbit Anti-DcR1 (Decoy Receptors for TRAIL, ED2, TRAIL-R3, TRID, LIT)

Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL. Two decoy receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain, but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts are expressed in many normal human tissues, but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control
HeLa cell lysate
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Raised against a peptide (CKEGTFRNENSPE) corresponding to amino acids 111 to 123 at extracellular domain of human DcR1 precursor.
Form
Supplied as a liquid in PBS with 0.02% sodium azide.
Purity
Chromatography purified IgG
Specificity
Recognizes human DcR1. An approximate 65kD band can be detected. Species crossreactivity: Mouse and rat..

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Pan, G., O’Rourke, K., Chinnaiyan, et al. Science; 1997; 276: 111–113. 2. Pan, G., Ni, J., Wei, Y.F., et al. Science; 1997; 277: 815–8. 3. Sheridan, J.P., Marsters, S.A., Pitti, R.M., et al. Science 1997; 277: 818–21. 4. Degli-Esposti, M.A., Smolak, P.J., Walczak, H., Smith, C.A., et al. J. Exp. Med. 1997; 186(7): 1,165–70. 5. MacFarlane, M., Ahmad, M., Srinivasula, S.M., et al. J. Biol. Chem. 1997; 272(41): 25,417–20. 6. Schneider, P., Bodmer, J.L., Thome, M., Hofmann, K., Holler, N., Tschopp, J., FEBS Lett 1997; 416(3): 329–34 7. Mongkolsapaya, J., Cowper, A.E., Xu, X.N., et al. J. Immunol. 1998; 160(1): 3–6. 8. Marsters, S.A., Sheridan, J.P., Pitti, R.M., et al. Curr. Biol. 1997; 7: 1,003–6. 9. Degli-Esposti, M.A., Dougall, W.C., Smolak, P.J., et al. Immunity 1997; 7: 813–20. 10. Pan, G., Ni, J., Yu, G., et al. FEBS Lett 1998; 424: 41–5.
USBio References
No references available
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