Mouse Anti-DC-SIGN (Dendritic Cell-specific ICAM3 grabbing Non-integrin, C Type Lectin Domain Family 4 Member L, CLEC4L, CD209, CD209 Antigen, CDSIGN, Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1, DC SIGN1, DC-SIGN1, HIV GP120 Binding Protein, MGC129965) (MaxLight 550)
CD209 Molecule
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
CD209 is involved in dendritic cell (DC) migration and the initial interaction between DC's and naive T lymphocytes. CD209 also binds HIV-1 gp120 and plays a key role in the dissemination of HIV-1 by DC's.
Applications
Suitable for use in Flow Cytometry and Immunofluorescence. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Immature myeloid monocyte-derived dendritic cells (MDDCs).
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), a 45kD C-type lectin that binds ICAM-3 that is also known as CD209. Reported to partially block the functional activity of DC-SIGN(2).
