D1550-03
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgGClone Number
5D7Grade
Affinity PurifiedApplications
IHCCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CMouse Anti-DC-SIGN, CT (Dendritic Cells, CD209, Dendritic Cell-Specific ICAM-3, SIGNR1, SIGN-R1)
Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes (1), where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC- SIGN (2). ICAM-3 is the natural ligand for DC-SIGN (3). A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC- SIGN2) was identified recently (4-8). DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR (8). The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.
SOURCE
Mouse monoclonal DC-SIGN antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DC-SIGN (1). DC-SIGN antibody was purified from ascites from hybridoma clone 5D7.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 5-10ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Jurkat cells
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide, DC-SIGN aa near the carboxy terminus of human DC-SIGN (1).
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DC-SIGN.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Geijtenbeek TB, et al., Cell 2000; 100:587-97. 2. Pohlmann S, et al., J. Virol. 2001;75:4664-72. |3. Geijtenbeek TB, et al.,. Cell 2000; 100:575-85. 4. Soilleux EJ, et al., J. Immunol. 2000 165:2937-42. |5. Pohlmann S, et al., Proc Natl Acad Sci USA. 2001; 98:2670-2675. 6. Bashirova AA, et al., J. Exp. Med. 2001; 193:671-8. 7. Mitchell DA, et al., J Biol Chem. 2001; 276:28939-45. 8. Mummidi S, et al., J Biol Chem. 2001; 276:33196-212.USBio References
No references available