Mouse Anti-DDB1 (Damage-specific DNA Binding Protein 1)
DNA damage binding protein (DDB) is a heterodimer that functions in DNA repair, and is associated with the DNA repair deficiency disease xeroderma pigmentosum (XP). The damaged DNA binding function of DDB requires both DDB1 (p125, 127kD) and DDB2 (p48, 48kD) subunits. When the subunits of DDB are expressed individually, p48 localized in the nucleus and p125 localized in the cytoplasm. Co-expression of p125 with p48 resulted in an increased accumulation of p125 in the nucleus, indicating that p48 plays a critical role in the nuclear localization of p125.
Applications
Suitable for use in Immunoprecipitation, Western Blot and ELISA. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Positive Control
Human HeLa cells
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human DDB1.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human DDB1 protein at ~130kD.
