D1575-30
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
WBCrossreactivity
Ca Hu Mo RtAccession #
NP_001017977Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-DCAF6 (DDB1- and CUL4-associated Factor 6, Androgen Receptor Complex-associated Protein, ARCAP, IQ Motif and WD Repeat-containing Protein 1, Nuclear Receptor Interaction Protein, NRIP, IQWD1, MSTP055)
IQ motif and WD repeats 1 (IQWD1) WD-repeat proteins are a large family of eukaryotic proteins coordinating multi-protein complex assemblies and their role has been implicated in multiple cellular processes including signal transduction, transcriptional regulation, cell cycle control and apoptosis. NRIP is a novel 860aa nuclear protein consisting of seven conserved WD40 domains and one NLS motif. It binds to androgen and glucocorticoid receptors and up regulates their transcriptional activity, thereby functioning as a nuclear receptor coactivator. Role of NRIP has been implicated in cell growth and also in cervical and prostrate cancer thus indicating a therapeutic intervention. Northern Blot analysis detected a high expression of NRIP in skeletal muscle and testis and low expression in heart, prostrate and adrenal gland.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa800-850 of human NRIP.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human NRIP. Species Crossreactivity: canine, mouse, rat. Species sequence homology: zebrafish, xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Tsai,T.C., Lee,Y.L., Hsiao,W.C., Tsao,Y.P. and Chen,S.L. NRIP, a novel nuclear receptor interaction protein, enhances the transcriptional activity of nuclear receptors. J. Biol. Chem. 280 (20), 20000-20009 (2005). 2. Tureci,O., Sahin,U., Koslowski,M., Buss,B., Bell,C., Ballweber,P., Zwick,C., Eberle,T., Zuber,M., Villena-Heinsen,C., Seitz,G. and Pfreundschuh,M. A novel tumour associated leucine zipper protein targeting to sites of gene transcription and splicing. Oncogene 21 (24), 3879-3888 (2002).USBio References
No references available