Mouse Anti-Dectin-1 (C-type Lectin Domain Family 7 Member A, Dendritic Cell-associated C-type Lectin 1, DC-associated C-type Lectin 1, Beta-glucan Receptor, CANDF4, C-type Lectin Superfamily Member 12, CLEC7A, BGR, CLECSF12, DECTIN1, UNQ539/PRO1082) (HRP)
Dectin-1, also known as beta-glucan receptor (ßGR), is a C-type lectin domain family member. It is a major pathogen pattern-recognition receptor that binds beta-glucans, glucose polymers that form cell-wall components or exopolymers of yeasts, fungi and some bacteria. Beta-glucans have also been used experimentally and therapeutically as immunomodulators that enhance resistance to bacterial, yeast, viral and protozoan infections, as well as tumor formation. Dectin-1 also acts as a co-stimulatory molecule on T-cells to induce their proliferation and is necessary for the TLR2-mediated inflammatory response. It is highly expressed on peripheral blood leukocytes and dendritic cells. Human Dectin-1 has two major (betaGR-A and betaGR-B) and several minor isoforms. BetaGR-A and betaGR-B differs by the presence and absence, respectively, of a stalk region.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Negative Control
I1904-84Q3: IgG2b Murine Negative Control
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
NIH3T3 cells expressing full length Dectin-1
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human Dectin-1 (beta-glucan receptor (betaGR)). Reacts with the betaGR-A isoform only.
