Rabbit Anti-Deoxyribonuclease I (DNase I)
Deoxyribonuclease I (DNase I) is a Ca 2+ and Mg 2+ dependent endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo-and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis.
Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Conjugation. Other applications have not been tested.
Recommended Dilution
ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:5000 Immunoprecipitation: 1:100 Optimal dilutions to be detemined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Bovine pancreatic Deoxyribonuclease I
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide. Reconstitute with 100ul sterile dH2O.
Purity
Purified by Ion Exchange chromatography.
Specificity
Recognizes 31kD bovine Deoxyribonuclease I. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum and well as purified and partially purified deoxyribonuclease I, (bovine pancreas).
