Technical Data

D3201-35
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Serum
Applications
E
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Deoxyribonuclease II (DNase II) (Not for Export EU)

Deoxyribonuclease II (Dnase II, EC.3.1.22.1) is one of the two Dnase present in mammalian tissues and biological fluids. Dnase II hydrolyzes DNA to 3'-phosphoryl oligonucleotides under acidic conditions. Dnase II is also known as "acid Dnase". It has preference of for dsDNA. Dnase II has been implicated in internucleosomal cleavage of chromatin to generate DNA fragments or ladder, a hallmark of apoptosis. Dnase II (mouse 353-aa; human 360-aa, chromosome 19p13.2-q13.4) is a lysosomal enzyme present in most tissues. Dnase Ii is synthesized as a pro-enzyme (signal peptide 1-16 aa, propeptide region of 91-aa, and 253-aa mature protein (110-353 aa) and processed to produce active enzyme containing 2-chains of ~32 and 12kD. Dnase II has no significant sequence homology with Dnase I.

Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000-50,000. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
A 17-aa peptide near the C-terminus of mouse Dnase II (1) was synthesized, conjugated to KLH.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Purity
Serum
Specificity
Recognizes mouse Deoxyribonuclease II (Dnase II). The peptide sequence is not well conserved in human Dnase II. No significant sequence homology with other lysosomal enzymes.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Yasuda T et al (1998) JBC 273, 2610-2616; Barker KP et al (1998) Gene 215, 281-289; Shilokawa D et al (1998) BBRC 247, 864-869; Yasuda T et al (1998) Ann. Hum. Genet. 62, 299-305
USBio References
No references available
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