Rabbit Anti-DERL3, NT (Degradation in Endoplasmic Reticulum Protein 3, DERtrin-3, Der1-like Protein 3, C22orf14, DER3, LLN2, Derlin-3)
Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal glycoproteins, but not that of misfolded nonglycoproteins. May act by forming a channel that allows the retrotranslocation of misfolded glycoproteins into the cytosol where they are ubiquitinated and degraded by the proteasome. May mediate the interaction between VCP and the degradation substrate.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1ug/ml Western Blot: 1ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 20ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human DERL3 at N-terminal (KLH).
Form
Supplied as a liquid in PBS, 0.01% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DERL3.
