D3221-05
Clone Type
MonoclonalHost
MouseSource
ChickenIsotype
IgG2bClone Number
4A44Grade
Affinity PurifiedApplications
IC IHC WBCrossreactivity
Ca Ch Hm Hu Mo Po Rb Rt ZeShipping Temp
Blue IceStorage Temp
-20°CNotes
BSA Free
Mouse Anti-Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)
Desmin (53kD) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of Desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal and cardiac muscle cells Desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of Desmin in smooth muscle is much more heterogenous. Coexpression of Vimentin and Desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard disfunction dramatic changes in the distribution of Desmin have been observed.
Applications
Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (Frozen): 1:100-1:200 using ABC method. Western Blot: 1:100-1:1000 Optimum dilutions to be determined by researcher.
Hybridoma
Sp2/0-Ag14 myeloma cells with spleen cells from a BALB/c mouse
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Cytoskeletal Desmin extract of chicken gizzard
Form
Supplied as a liquid in PBS, 0.09% sodium azide
Purity
Purified by Protein G affinity chromatography
Specificity
Recognizes chicken Desmin, which is expressed in smooth and striated muscle cells and their tumors, i.e. rhabdomyosarcoma and leiomyosarcoma. Species Crossreactivity: human, mouse, rat, hamster, canine, porcine, rabbit and zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Pieper, F.R., et al., J. Cell Biol. 108: 1009-1024 (1989). 2. Raats, J.M.H., et al., J. Cell Biol. 111: 1971-1985 (1990). 3. Schaart, G., et al., Development 107: 585-596 (1989). 4. Quax, W., et al., Cell 43: 327-338 (1985). 5. Verhagen, A.P., et al., Prostate 13: 25-38 (1988). 6. Schaart, G., et al., Differentiation 46: 105-115 (1991). 7. Dispersyn, G.D., et al., Cardiovasc. Res. 51: 230-240 (2001). 8. Council, L. and Hameed, O., Modern Pathology 22: 639-650 (2009).USBio References
No references available