Technical Data

D3575-55A
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2b,k
Clone Number
33CT11.40.3
Grade
Ascites
Applications
E WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-DRP-2 (Dihydropyrimidinase Related Protein-2, CRMP-2, Collapsin Response Mediator Protein 2,N2A3, Unc-33-like Phosphoprotein 2, ULIP-2, DPYSL2, CRMP2, ULIP2)

DRP-2, also known as collapsin response mediator protein-2 (CRMP-2), is expressed at high levels in the developing nervous system and plays a critical role in axonal outgrowth by specifying axon/dendrite fate and establishing neuronal polarity (1,2). CRMP-2 enhances axon elongation and branching by binding to tubulin heterodimers to promote microtubule assembly (3). GSK-3b inactivates CRMP-2 by phosphorylating it at Thr514. CRMP-2 is primed following phosphorylation at Ser522 by CDK5 and at Thr518 by GSK-3b (2). Phosphorylation of CRMP-2, which decreases tubulin binding ability, can be inhibited by NT-3 and BDNF through the PI3 kinase/Akt pathway (2). CRMP-2 also mediates semaphorin-induced growth cone collapse (4). Hyperphosphorylation of CRMP-2 and is found in Alzheimer disease plaques with concurrent elevated GSK-3b activity in these patients (5).

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:500-1:1,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant DHP fusion protein.
Form
Supplied as a liquid, 0.09% sodium azide.
Purity
Ascites
Specificity
Recognizes human DRP-2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hamajima,N., Gene 180 (1-2), 157-163 (1996).
USBio References
No references available
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