D3810
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
WBCrossreactivity
Hu MoShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Dkk-1 (DKK1, SK, DKK-1, Dickkopf Homolog 1 (Xenopus laevis), Dickkopf-1 Like, Dickkopf (Xenopus laevis) Homolog 1)
The human Dickkopf (Dkk)-related proteins are a structurally and functionally heterogeneous family of proteins. This family is comprised of hDkk1, -2, -3, -4 and Soggy (Sgy), a Dkk-3 related protein. Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues and are secreted when expressed in 293T cells. Several secreted antagonists, including Dickkopf-1 (Dkk-1) (German for “big head, stubborn”) regulate the activity of Wnt proteins secreted glycoproteins that influence many aspects of embryonic development in metazoans. Dkk-1 is a head inducer secreted from the vertebrate head organizer, that induces anterior development of Xenopus by antagonizing Wnt signaling. Dkk-1 inhibits Wnt-induced axis duplication, acts upstream of disheveled3 and antagonizes Wnt signaling upstream of its effect on beta-catenin regulation. Dkk-1 is an LRP6 ligand5 that inhibits Wnt signaling by blocking Wnt-induced Fz-LRP6 complex formation. Dkk-1 plays a crucial role in the control of programmed cell death during vertebrate limb development. Bmp-4 is one of several upstream regulators of Dkk-1 expression. Furthermore, the proapoptotic hDkk-1 gene may play important roles in linking the oncogenic Wnt and p53 tumor suppressor pathways.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide derived from the C-terminal region of the human and mouse Dkk-1(Dickkopf-1) proteins.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human and mouse Dkk-1 proteins. On Western blots, it identifies the target band at ~35kD. Reactivity has been confirmed with human Dkk-1-transfected 293T cell lysates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Krupnik VE, et al. Gene 238(2):301-313, 1999.2. Niehrs C, et al. Int J Dev Biol 45(1):237-240, 2001.3. Glinka A, et al. Nature 391(6665):357-362, 1998.4. Fedi P, et al. J Biol Chem 274(27):19465-19472, 1999.5. Balfico A, et al. Nat Cell Biol 3(7):683-686, 2001.6. Semenov MV, et alCurr Biol 11(12):951-961, 2001.7. Grotewold L & Ruther U. Int J Dev Biol 46(7):943-947, 2002a.8. Grotewold L & Ruther U. EMBO J 21(5):966-975, 2002b.9. Shou J, et al. Oncogene 21(6):878-889, 2002.USBio References
No references available