Technical Data

D3931-63A
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Purified
Applications
WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-DNA Methyltransferase 2 (Dnmt 2)

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.

Applications
Can be used for detection of mouse and human Dnmt2 by Western blotting. Western blot analysis for Dnmt2a using antibody at 2ug/ml dilution against 10ug of mouse testes lysates. A protein band of approximate mol. wt. of 42kD is detected.
Recommended Dilution
Western blot: 2ug/ml
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptides corresponding amino acids 39-53 and 361-376 of mouse Dnmt2.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified
Specificity
Recognizes both mouse and human Dnmt2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Yoder JA, Bestor TH. Hum Mol Genet 7(2):279-84 (1998). 2. Okano M, Xie S, Li E.: Nucleic Acids Res 26:2536-40 (1998). |
USBio References
No references available
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