Technical Data

D8080-01K
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-DOK1, NT (Docking Protein 1, Downstream Of Tyrosine Kinase 1, p62(dok), pp62)

DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. DOK2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the N-terminal region of human DOK1 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human DOK1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Liang, X., et al., J. Biol. Chem. 277(16):13732-13738 (2002). 2.Yamakawa, N., et al., EMBO J. 21(7):1684-1694 (2002). 3.Hubert, P., et al., Eur. J. Immunogenet. 27(3):145-148 (2000). 4.Nemorin, J.G., et al., J. Biol. Chem. 275(19):14590-14597 (2000). 5.Nelms, K., et al., Genomics 53(2):243-245 (1998).
USBio References
No references available
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