Goat Anti-Dok5 (Docking Protein 5, C20orf180, dJ805C22.1, Downstream of Tyrosine Kinase 5, IRS6, MGC16926, Protein Dok-5)
chromosome 20 open reading frame 180, downstream of tyrosine kinase 5, docking protein 5, C20orf180, MGC16926, DOK5
The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. In contrast to other DOK family proteins, this protein does not interact with RASGAP.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Peptide ELISA Titer: 1:16,000 Western Blot: 1-3ug/ml In transfected HEK293 transiently expressing DOK5 a band of ~38kD is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 35.5kD according to NP_060901.2. Immunohistochemistry: 1-2ug/ml In paraffin embedded tonsil, stains scattered cells in the interfollicular area compatible with mast cells. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to CLHRTETFPAYRSEH, from the C Terminus of human Dok5 (NP_060901.2).
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes human Dok5. Species sequence homology: mouse, rat, canine.
