Rabbit Anti-DRAK2 (DAP Kinase-related Apoptosis-inducing Protein Kinase 2)
DRAK2 (DAP kinase related apoptosis inducing protein kinase 2) is a member of the novel DAP (death associated protein) pro-apoptotic kinase family (1). Overexpression of DRAK2 in NIH/3T3 cells induces morphological changes associated with apoptosis, which are likely to occur in a p53-dependent manner (1,2). DRAK2 is preferentially expressed in lymphoid tissues and regulates the TCR activation threshold during thymocyte selection (3). Indeed, T cells from DRAK2(-/-) mice exhibit enhanced sensitivity to T cell receptor-mediated stimulation and have a reduced requirement for co-stimulation (4).
Applications
Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:200 Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (paraffin): 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding Asp315 of mouse DRAK2.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Specificity
Recognizes endogenous levels of total mouse DRAK2 protein.