Technical Data

D9335-01J
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Accession #
NP_004217.1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-DRAK2 (STK17B, Serine/threonine kinase 17b, DAP kinase-related apoptosis-inducing protein kinase 2)

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases. DRAKs contain an N-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:32000 Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-HSVRSSEDKTSK, from the internal region of thuman DRAK2 (NP_004217.1).
Form
Supplied as a liquid in Tris saline, pH7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DRAK2. Sequence Homology: canine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Friedrich ML, Cui M, Hernandez JB, Weist BM, Andersen HM, Zhang X, Huang L, Walsh CM.|Modulation of DRAK2 autophosphorylation by antigen receptor signaling in primary lymphocytes.|J Biol Chem. 2007 Feb 282 (7): 4573-84. PMID: 17182616
USBio References
No references available
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