Technical Data

D9452-02
Clone Type
Monoclonal
Host
Mouse
Isotype
IgG1
Clone Number
5F271
Grade
Ascites
Applications
IF IHC IP WB
Crossreactivity
Bo Ca Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Drebrin (D0S117E, Drebrin 1, DBN 1, DBN1, Developmentally Regulated Brain Protein, DKFZp434D064, Drebrin E)

Drebrin is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells. Positive staining is found in many tissues, including diverse epithelia and carcinoma (e.g. in epidermis basal cells are positive, whereas overlying cells are negative; also positive: regenerating epithelium during wound healing; basal cell carcinoma; skin melanoma), specific types of endothelia and smooth muscle; especially prominent in the mesangial cells of renal glomeruli and in the Sertoli cells of testis. Also positive: epithelia of hair follicles and eccrine sweat glands. Hepatocytes and cross-striated muscle consistently stain negative.

Applications
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (frozen tissue only): 1:100 with PBS, pH 7.4; Incubation time 1 hr at RT. Optimal dilutions to be determined by the researcher.
Recommended Positive Control
Primary human keratinocytes, U333, MCF-7, SV80; B16 (mouse melanoma cells)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa632-649 from the C-terminal, of Derbrin conjugated to KLH.
Form
Supplied as a liquid, 0.09% sodium azide.
Purity
Ascites
Specificity
Recognizes drebrin, a widespread actin-associating protein of 70kD (SDS-PAGE mobility shows Mr 120,000). Species Crossreactivity: human, bovine, canine, rat and mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Peitsch WK, Grund C, Kuhn C, Schnölzer M, Spring H, Schmelz M, Franke WW: Drebrin is a widespread actin-associating protein enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells. Eur J Cell Biol 78: 767-778 (1999). 2. Peitsch WK, Hofmann I, Bulkescher J, Hergt M, Spring H, Bleyl U, Goerdt S, Franke WW: Drebrin, an actin-binding, cell-type characteristic protein: Induction and localization in epithelial skin tumors and cultured keratinocytes. J Invest Dermatol 125, 761-774 (2005).
USBio References
No references available
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