Mouse Anti-DSCAM (Down Syndrome Cell Adhesion Molecule)
The Dscam family of cell surface proteins mediates recognition events between neurons that play an essential role in the establishment of neural circuits in many organisms. Interneurons form synaptic connections with retinal ganglion cells in specific layers. Dscam and DscamL are expressed in different subsets of interneurons and ganglion cells. Interestingly, interneurons and ganglion cells that target to the same layer, express the same Dscam protein.
Applications
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Recommended Dilutions
Western Blot: 0.5ug/ml Immunohistochemistry: 1:250-1:500 Optimal dilutions to be determined by the researcher.
Positive Control
Transfected HEK293 cell lysate.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to chicken Dscam (Histidine-tagged).
Form
Supplied as a lyophilized powder with no additives. Reconstitute with 100ul ddH2O.
Specificity
Recognizes chicken Dscam. Species Crossreactivity: mouse.