Technical Data

D9807-19
Grade
Cell Culture Grade
Shipping Temp
RT
Storage Temp
RT/4°C
Anti-Dulbecco’s MEM (DMEM) F-12 w/o Choline Chloride, Phenol Red

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Appearance
White to off-white, homogenous, free flowing powder
Solubility
Colorless to light yellow, clear, complete
pH
As Reported
Endotoxin
≤1EU/ml

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Gibb AA, et al. Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes. Biochem J. 2017 Jul 13. pii: BCJ20170474. doi: 10.1042/BCJ20170474. 2. Biesemann N., et al. High throughput screening of mitochondrial bioenergetics in human differentiated myotubes identifies novel enhancers of muscle performance in aged mice. 2018. Sci Rep. 8(1):9408. doi: 10.1038/s41598-018-27614-8.
USBio References
1. Gibb AA, et al. Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes. Biochem J. 2017 Jul 13. pii: BCJ20170474. doi: 10.1042/BCJ20170474. 2. Biesemann N., et al. High throughput screening of mitochondrial bioenergetics in human differentiated myotubes identifies novel enhancers of muscle performance in aged mice. 2018. Sci Rep. 8(1):9408. doi: 10.1038/s41598-018-27614-8.
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