D9840-08A
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
PurifiedApplications
E IHC WBCrossreactivity
Hu MoAccession #
NP_001385Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-DUSP4, CT (Dual Specificity Protein Phosphatase 4, Dual Specificity Protein Phosphatase hVH2, HVH2, Mitogen-activated Protein Kinase Phosphatase 2, MAP Kinase Phosphatase 2, MKP2, MKP-2, TYP, VH2)
Dual Specificity Phosphatase 4, EC=3.1.3.16, EC=3.1.3.48
DUSP4 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP4 inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 331-361 in the C-terminal region of human DUSP4, UniProt Accession #Q13115.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human DUSP4. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1.Chen, P., et al., J. Biol. Chem. 276(31):29440-29449 (2001). 2.Smith, A., et al., Genomics 42(3):524-527 (1997). 3.Chu, Y., et al., J. Biol. Chem. 271(11):6497-6501 (1996). 4.King, A.G., et al., Oncogene 11(12):2553-2563 (1995). 5.Guan, K.L., et al., J. Biol. Chem. 270(13):7197-7203 (1995).USBio References
No references available