Technical Data

D9840-30J
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Bo Hu Mo Rt
Accession #
NP_004411.2
Gene ID
1850, 18218
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-DUSP8 (Dual Specificity Protein Phosphatase 8, Dual Specificity Protein Phosphatase hVH-5, VH5)

DUSP8 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP8 inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:4000 Western Blot: 1-3ug/ml, human heart lysate observed on ~60-65kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to AGDRLPRKVMDAK-C, from human DUSP8, at N-terminal (NP_004411.2).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DUSP8. Species sequence homology: Bovine, mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Martell KJ, Seasholtz AF, Kwak SP, Clemens KK, Dixon JE. hVH-5: a protein tyrosine phosphatase abundant in brain that inactivates mitogen-activated protein kinase. J Neurochem. 1995 Oct;65(4):1823-33.
USBio References
No references available
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