Rabbit Anti-DUSP16 (Dual Specificity Protein Phosphatase 16, KIAA1700, Mitogen-activated Protein Kinase Phosphatase 7, MAP Kinase Phosphatase 7, MKP7, MKP-7)
Dual Specificity Phosphatase 16, EC=3.1.3.16, EC=3.1.3.48, FLJ36298, FLJ40991, MGC129701, MGC129702
DUSP16 is a 665aa novel mitogen-activated protein kinase phosphatase belonging to the non-receptor class of the protein-tyrosine phosphatase family with a rhodanese-like domain, protein tyrosine phosphatase catalytic domain and a long terminal stretch containing nuclear export and localization signal. DUSP16 are dual-specificity phosphatases that dephosphorylate the TXY motif of MAPK TXY motifs, thereby inactivating MAP kinases. DUSP16 is ubiquitously expressed and behaves both as a nuclear shuttle and as a phosphatase. It binds to and inactivates p38 MAPK and JNK/SAPK, p38 alpha and -beta but not gamma or delta and ERK.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1-3ug/ml Immunohistochemistry (paraffin): 10ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Jurkat cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa600-650 of human DUSP16.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DUSP16. Species Crossreactivity: chimpanzee, bovine, monkey, mouse. Species sequence homology: zebrafish, chicken.
