Technical Data

E2211-50D-ML650
Clone Type
Monoclonal
Host
Mouse
Source
Mouse
Conjugate
MaxLight™650
Isotype
IgG2a,k
Clone Number
AA19
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo
Accession #
NP_068676
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-EED (Embryonic ectoderm development, hEED, HEED, Polycomb protein EED, WAIT1, WAIT-1, WD protein associating with integrin cytoplasmic tails 1) (MaxLight 650)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Positive Control
A431 Cell Lysate
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant fusion protein corresponding to full length mouse EED, tagged with MBP.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse EED (5-6 isoforms of EED are detected). Species Crossreactivity: human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. The Protein Data Bank, www.pdb.org, DOI 10.2210/pdb2qxv/pdb 2. Pasini, D., et al. (2007). Mol. Cell. |Biol. 27, 3769-3779. 3. Bracken, A.P., et al. (2003). EMBO J. 22: 5323-5335. 4. Pasini, D., et al. (2004). 5. Bracken, A.P., et al. (2007). Cell Cycle. 3: 396-400 6. Bracken, A.P., et al. (2006). Genes Dev. 20: 1123-1136.
USBio References
No references available
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