Mouse Anti-EHD2 (EH Domain Containing 2, FLJ96617, PAST2) (Azide Free)
EH-domain Containing Protein 2
This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.
Applications
Suitable for use in ELISA, Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:500-1:1000 ELISA: 1ug/ml-3ng/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to a portion of amino acids within aa365-428 of human EHD20 (GST tag) (NP_055416).
Form
Supplied as a liquid in PBS, pH 7.2. Azide free.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human EHD2 at ~61kD.
