Technical Data

E2240-10D
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_005220
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ELK1 (S383) (ETS Domain-containing Protein Elk-1)

ELK1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein is a nuclear target for the ras-raf-MAPK signaling cascade.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to amino acid residues surrounding S383 of human ELK1 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ELK1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Salinas, S., et al., J. Cell Biol. 165(6):767-773 (2004). 2.Yang, S.H., et al., Mol. Cell 12(1):63-74 (2003). 3.Bebien, M., et al., Oncogene 22(12):1836-1847 (2003). 4.Sharrocks, A.D., Biochem. Soc. Trans. 30(2):1-9 (2002). 5.Murai, K., et al., Mol. Cell. Biol. 22(20):7083-7092 (2002).
USBio References
No references available
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