Technical Data

E2249-82A
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_060561
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ELP3, CT (Elongator Complex Protein 3, EC 2.3.1.48, hELP3)

Elongator complex protein 3 (ELP3) is a catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. It may also have a methyltransferase activity.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human ELP3 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human ELP3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Han,Q., Acta Biochim. Biophys. Sin. (Shanghai) 39(6), 453-461 (2007) 2.Kim,J.H., Proc. Natl. Acad. Sci. USA 99(3), 1241-1246 (2002) 3.Ninomiya,Y., J. Biochem. 118(2), 380-389 (1995).
USBio References
No references available
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