Mouse Anti-Endoglin (CD105, END, Endoglin, FLJ41744, HHT1, ORW, ORW1)
Endoglin is a homodimeric integral membrane glycoprotein comprised of 95kD subunit. Endoglin is a component of the TGF-B receptor system that functions as an ancillary receptor influencing binding of the Transforming Growth Factor beta (TGF-B) family ligands to signaling receptors. Endoglin binds TGF-B1 and TGF-B3 with high affinity but does not bind TGF-B2. Endoglin is expressed by vascular endothelial cells and syncytiotrophoblasts of placenta and is weakly expressed by stromalfibroblasts. It is also expressed by U937 cells and activated macrophages. Endoglin expression is up regulated on activated endothelium in tissues undergoing angiogenesis, such as in tumors, or in cases of wound healing or dermal inflammation.
Applications
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 detected Endoglin on 10ug of Jurkat lysates Immunohistochemistry: For use on acetone or paraformaldehyde fixed tissue. Optimal dilutions to be determined by the researcher.
Positive Control
Jurkat cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human resting umbilical vein endothelial cells. |Cellular Localization: Membrane; Single-pass type I membrane protein. Species Sequence Homology: rat
Form
Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide
Specificity
Recognizes human endoglin. Species Crossreactivity: mouse.
