Mouse Anti-Enolase 2 (ENO2, Isoenzyme Neuron-specific Enolase)
Enolase 2 (2-phospho-D-glycerate hydrolyase; also neural enolase and Y-enolase) is a 46kD member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434aa in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with a-enolase. Full-length human Enolase 2 is 99% aa identical to both mouse and canine Enolase 2. It shares 83% aa identity with human enolases # 1 and # 3.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Immunoprecipitation: 25ug/ml Western Blot (Non-reducing): 2ug/ml Immunohistochemistry: 25ug/ml Optimal dilutions to be determined by the researcher.
Hybridoma
Mouse myeloma cells with B cells obtained from a mouse immunized with purified, E.coli-derived, recombinant human Enolase 2 (aa1-434).
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Met1-Leu434 of human Enolase 2 expressed in E. coli. Species sequence homology: mouse, canine (99%).
Form
Supplied as a lyophilized powder from PBS, pH 7.0, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human Enolase 2.
