Technical Data

E3224-07
Clone Type
Polyclonal
Host
Sheep
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC IP WB
Crossreactivity
Mo
Accession #
NP_001025462
Shipping Temp
Blue Ice
Storage Temp
-20°C
Sheep Anti-Ectonucleotide Pyrophosphatase, Phosphodiesterase 7 (ENPP-7, Gm254, Alk-SMase, Enpp7)

ENPP7 is a member of the ecto-nucleotide pyrophosphatase/ phosphodiesterase (NPP) family with specific activity against lipids with positively charged phosphocholine headgroup including sphingomyelin, lysophosphatidylcholine and platelet activating factor (PAF). It hydrolyzes sphingomyelin to generate ceramide, a potent antiproliferative and proapoptotic molecule. It hydrolyzes lysophosphatidylcholine to monoglyceride and therefore competes with lysophospholipase D to reduce the formation of lysophosphatidic acid, a potent factor for inflammation and angiogenesis. It hydrolyses PAF to 1-0-alkyl-2-acetyl-sn-glycerol and inhibits PAF-induced inflammatory responses. ENPP7 has been proposed as a tumor suppressor protein. In addition, ENPP7 may influence cholesterol absorption by hydrolyzing sphingomyelin in the intestinal lumen and on the apical surface of microvilli, as the levels of sphingomyelin in the intestinal tract affect cholesterol absorption.

Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Immunoprecipitation. Other applications not tested.
Recommended Dilution
ELISA: 0.5-1ug/ml Western Blot: 1ug/ml Immunohistochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
NS0-derived, recombinant mouse ENPP-7.
Form
Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse ENPP-7. Species sequence homology: human (50%).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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