Technical Data

E3225-01
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Accession #
NP_004946
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ENT1, CT (Equilibrative Nitrobenzylmercaptopurine Riboside-sensitive Nucleoside Transporter, Equilibrative NBMPR-sensitive Nucleoside Transporter,Nucleoside Transporter, es-type, Solute Carrier Family 29 Member 1, Slc29a1, Equilibrative Nucleoside Transporter 1)

ENT1 is a member of the equilibrative nucleoside transporter family. It is a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to a region between corresponding to a portion of amino acids within aa 402-431 from the C-terminal region of human ENT1, conjugated to KLH. Species sequence homology: mouse, rat
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ENT1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Bone,D.B.,Am. J. Physiol. Heart Circ. Physiol. 293(6), H3325-H3332 (2007) 2.Damaraju,V.L., Am. J. Physiol. Renal Physiol. 293(1), F200-F211 (2007) 3.Abdulla,P.,Nucleosides Nucleotides Nucleic Acids 26(1), 99-110 (2007) 4.Sundaram,M., J. Biol. Chem. 276(48), 45270-45275 (2001).
USBio References
No references available
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