Rabbit Anti-Ephrin A1 (ECKLG, EFL1, EPH-related receptor tyrosine kinase ligand 1, Ephrin-A1, EPLG1) (PE)
Ephrins are the ligands which bind to the Eph receptor subfamily, the largest group of the receptor tyrosine kinase family. Both ephrins and Eph receptors are broadly expressed throughout the ectoderm, mesoderm, and endoderm of vertebrate embryos.1 The interaction of ephrins with the appropriate Eph receptor(s) has been implicated in a wide spectrum of functions in vertebrate morphogenesis, including the accurate migration of embryonic cells,2 formation of boundaries between structures (i.e. rhombomeres and somites), and the control of cytoskeletal changes which dictate cellular shape and adhesion during development. Two classes of ephrins exist: Class A ephrins are tethered to the membrane by a GPI linkage and bind primarily to EphA receptors; Class B ephrins contain a membrane-spanning region and bind predominantly to EphB receptors. Ephrin-A1, also known as B61, LERK-1, and EFL1,3 is a Class A protein. It has been shown to bind the EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, and EphB2 receptors.
Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1-5ug/ml Immunoprecipitation: 5-10ug/IP reaction Immunohistochemistry: 4-10ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide derived from the C-terminal end of mouse ephrin-A1 protein.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes the mouse ephrin-A1 protein. Reactivity is confirmed with a chimeric protein consisting of the extracellular domain of mouse ephrin-A1 and the Fc region of human IgG1. Crossreactivity with human ephrin-A1 is confirmed with Immunohistochemistry experiments on human tissue sections and this reactivity was expected because of the 85% shared amino acid identity in the extracellular domain.
