Rabbit Anti-Ephrin A4 (EFNA4) (FITC)
Ephrins are the ligands which bind to the Eph receptor subfamily, the largest group of the receptor tyrosine kinase family. Both ephrins and Eph receptors are broadly expressed throughout the ectoderm, mesoderm, and endoderm of vertebrate embryos. The interaction of ephrins with the appropriate Eph receptor(s) has been implicated in a wide spectrum of functions in vertebrate morphogenesis, including the accurate migration of embryonic cells, formation of boundaries between structures (i.e. rhombomeres and somites), and the control of cytoskeletal changes which dictate cellular shape and adhesion during development. Two classes of ephrins exist: Class A ephrins are tethered to the membrane by a GPI linkage and bind primarily to EphA receptors; Class B ephrins contain a membrane-spanning region and bind predominantly to EphB receptors. Ephrin-A4, also known as LERK-4 and EFL-4, is a Class A protein. It has been shown to bind the EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 receptors.
Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1-5ug/mL Immunoprecipitation: 5-10ug/ IP reaction Immunohistochemistry (FFPE): 4-10ug/ml. Heat induced epitope retrieval with EDTA, pH 8.0 was required for optimal staining. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide derived from the C-terminal end of the mouse ephrin-A4 protein.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse ephrin-A4 protein. Reactivity is confirmed with a chimeric protein consisting of the extracellular domain of human ephrin-A4 and the Fc region of human IgG1. Crossreactivity with human ephrin-A4 is also confirmed with IHC experiments on human tissue sections and this reactivity was expected because of the 80% shared amino acid identity in the extracellular domains.
