Rat Anti-EphB2 (Ephrin Type-B Receptor 2, Neural Kinase, Nuk Receptor Tyrosine Kinase, Tyrosine-protein Kinase Receptor EPH-3, Tyrosine-protein Kinase Receptor SEK-3, Epth3, Nuk, Sek3) (PE)
EphB2, also known as Cek5, Nuk, Erk, Qek5, Tyro5, Sek3, Hek5, and Drt, is a 125kD member of the transmembrane Eph receptor tyrosine kinase family. The 90kD extracellular domain can be shed from neurons by calcium ionophores or NMDA receptor agonists. The interaction between EphB2 and Ephrin-A5 promotes axonal growth cone collapse, while its interactions with Ephrin-B ligands are required for inner ear, renal, urorectal, and vascular development. Within the ECD, mouse EphB2 shares 99% aa sequence identity with human and rat EphB2.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 10ul/10e6 using COLO 205 human colorectal adenocarcinoma cell line Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Recombinant protein corresponding to Val27-Lys548 from mouse EphB2 expressed in NS0 cell line.
Form
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes mouse EphB2. Species Crossreactivity: human.
